You can compute sequence statistics, such as codon frequency and atomic composition of a protein, and interactively explore the sequences in the Sequence Viewer app. Reverse Complement: Convert a DNA sequence into its reverse, complement, or reverse-complement. Perform in-depth analyses of amino acid sequences to get better understanding of sequence features and functions. Loading concentrations have been optimized for staining with SYPRO Ruby, Silver Stain Plus™, or Bio-Safe™ Coomassie Blue. DNA Molecular Weight: Calculate the molecular weight of DNA single-stranded sequences are counted with a 5-P. If you want a plot of the relationship between charge and pH use ProteinChemist (ProteinChemist. These plug protein standards are unstained. Isoelectric Point - Compute pI/Mw tool (ExPASy, Switzerland). These plugs can be used on vertical 2-D gels with no reference well. point (pi) and molecular weight (MW) using simply the amino acid sequence of the protein would be extremely valuable to researchers who use two-dimensional. They come in easy-to-use snap-off molds in packs of 24 (one application per plug), allowing quick and clean loading of molecular weight standards on any gel.
Calculate pi and molecular weight with protein sequence plus#
Precision Plus Protein Standards are available cast into 1 mm thick agarose plugs for easy storage, handling, and loading. No dilution is required.Ĭonstituent Proteins of 2–D SDS-PAGE Standards Protein They consist of seven reduced, denatured proteins that can be visualized with silver or Coomassie Blue staining. These unique protein standards provide calibrated references for the pI and molecular weight of proteins in 2-D SDS-PAGE applications. For calibration of IEF tube gels containing urea, use 2-D SDS-PAGE standards. Paste the raw sequence or one or more FASTA sequences into the text area below. Use Protein Isoelectric Point when you want to know approximately where on a 2-D gel a particular protein will be found. * Because the IEF protein standards are in native form, they cannot be used with reducing or denaturing agents such as urea, β-mercaptoethanol, or dithiothreitol. Protein Isoelectric Point calculates the theoretical pI (isoelectric point) for the protein sequence you enter. These standards are provided in a stable aqueous solution, and need no reconstitution or dilution prior to use. Five of the nine proteins are naturally colored to aid in monitoring the focusing. Based on the amino acid sequence of myoglobin and the assumption that the pKa of the side chains are the same in the protein as for isolated amino acids, the calculated average net charges of apoMb would be approximately +30 at pH 3.5, +20 at pH 4.5, +9 at pH 6, and 0 at pH 7.8 (the calculated pI). Our IEF protein standards are a mixture of nine native proteins with pI ranging from 4.45 to 9.6. Isoelectric focusing (IEF) protein standards allow dependable and reproducible pI calibration in native polyacrylamide or agarose IEF gels.
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